Describe in great detail the steps in performing a Gram stain. Include incubation times. Deﬁne primary stain and counter stain. In the Gram stain, what dye is primary stain, which is the counter stain?
Describe the molecular basis behind Gram staining. What part of the bacteria is stained with the primary stain, what part is stained with the counter stain? Why do we wash alcohol over the slide before the counter stain?
What does it mean to be Gram positive, Gram negative?
21. What is the stain is shown in the picture below? What is stained green, what is stained red?
22. Describe in great detail the steps in performing an endospore stain. Include incubation times.
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